Calculadora de Dilución

Ingresa concentración inicial, volumen deseado y concentración final para calcular el volumen necesario. Soporta dilución serial, múltiples unidades de concentración y preparación de soluciones de laboratorio.

C₁ × V₁ = C₂ × V₂

Concentration Unit

Volume Unit

C₁ (Initial Concentration)

V₁ (Initial Volume)

10 mL

C₂ (Final Concentration)

V₂ (Final Volume)

Result

C₁:10 mM

V₁:10 mL

C₂:1 mM

V₂:100 mL

Dilution Factor:1:10

Solvent to Add:90 mL

Pipetting Diagram

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What is a Dilution Calculator?

A dilution calculator solves the equation C1V1 = C2V2, the fundamental formula for preparing solutions of known concentrations in chemistry, biology, and laboratory science. C1 is the initial (stock) concentration, V1 is the volume of stock needed, C2 is the desired final concentration, and V2 is the desired final volume. This tool also supports serial dilutions — a technique where a solution is progressively diluted in equal steps to create a range of concentrations, commonly used in microbiology assays, dose-response curves, ELISA, PCR standard curves, and antibiotic susceptibility testing.

How to Use This Calculator

Choose Simple Dilution or Serial Dilution mode
For simple dilution: enter any 3 of the 4 values (C1, V1, C2, V2) and select which to solve for
Select appropriate concentration units (M, mM, µM, nM, mg/mL, etc.) and volume units (L, mL, µL)
View the calculated result with the interactive pipetting diagram showing the dilution workflow
For serial dilution: set initial concentration, dilution factor, number of steps, and transfer/total volumes to generate the full dilution series with tube diagram

Frequently Asked Questions

What is the C1V1=C2V2 formula used for?

The C1V1=C2V2 equation (also known as the dilution equation) is used whenever you need to prepare a less concentrated solution from a more concentrated stock solution. It's fundamental in chemistry, molecular biology, pharmacology, and clinical labs for preparing reagents, buffers, drug dilutions, and standard curves.

How do I calculate a serial dilution?

A serial dilution involves taking a fixed volume from one solution and adding it to a fixed volume of diluent, then repeating the process for each subsequent tube. For example, a 1:10 serial dilution transfers 100 µL into 900 µL of diluent at each step, reducing the concentration by 10-fold each time. Our calculator generates the complete series with concentrations and volumes for each step.

What is the difference between dilution factor and dilution ratio?

A dilution factor of 10 (or 1:10 dilution) means the final concentration is 1/10 of the original. The dilution ratio 1:10 means 1 part sample to 9 parts diluent (total 10 parts). For serial dilutions, the cumulative dilution factor is the product of individual dilution factors at each step.

Can I use different concentration units for C1 and C2?

In this calculator, C1 and C2 share the same concentration unit to ensure consistency. If you need to convert between units (e.g., from molarity to mg/mL), you'll need to convert one value first using molecular weight. The calculator supports 9 concentration units including M, mM, µM, nM, mg/mL, µg/mL, ng/mL, %, and X (fold).